building 4x 4090 rig

I am currently using 2x 4090 rig which is simply attached via PCIex 4.0 and 5.0 slots. I suppose both x16. 

 planning to reassamble those GPUs into 4x 4090 rig on minig motherboard. 


I have question, especially to people who have 3090 rigs.  Is it so important , that GPUs will be connected via PCIex x1, will it result in great speed loss ? 
Or I could connect them via PCIex X1 and use for hashcat with no problem ?


P.s using 2x 4090 for hashcat mode 400 for long time, and totally satisfied with it